Table 1 Primers and PCR efficiencies of the candidate reference and target genes used for OP samples.
From: Postmenopausal Osteoporosis reference genes for qPCR expression assays
Name | Gene | Protein function | Primer sequences/Taqman® probe reference | Product size/bp | R2 | E/% |
|---|---|---|---|---|---|---|
G6PD | Glucose-6-phosphate 1-dehydrogenase | fatty acid and nucleic acid synthesis | F: CCGTGATGAGAAGGTCAAGGT R: TACTGGCCCAGGACCACATT | 72 | 0.999 | 95.12 |
B2M | Beta-2-microglobulin | small subunit of the MHC1 | F: TGAGTGGCATGAAGAAGGTGT R: GGCAGTTCTTTGCCCTCTCT | 77 | 0.999 | 104.79 |
GUSB | Beta-glucuronidase | degradation of dermatan and keratan sulfates | F: CACTGTGGCTGTCACCAAGA R: TCCGCATCCTCATGCTTGTT | 84 | 1 | 100.51 |
HSP90 | Heat shock protein HSP 90-beta | regulation of proteins in cell cycle control and signal transduction. | F: GCCTACTTGGTGGCAGAGAA R: CAGCAGAAGACTCCCAAGCA | 79 | 0.999 | 98.38 |
EF1A | Elongation factor 1-alpha 1 | protein synthesis | F: GAGGCTGCTGAGATGGGAAA R: CGTTCACGCTCAGCTTTCAG | 74 | 1 | 102.37 |
RPLP0 | 60 S acidic ribosomal protein P0 | ribosomal protein lateral stalk subunit P0 | F: GCGACCTGGAAGTCCAACTA R: TCTGCTTGGAGCCCACATTG | 100 | 0.999 | 102.02 |
GAPDH | Glyceraldehyde- 3-phosphate dehydrogenase | glycolytic enzyme | F: CTGATGCCCCCATGTTCGT R: GCAGGAGGCATTGCTGATGA | 80 | 0.999 | 96.81 |
ACTB | Actin, cytoplasmic 1 | cytoskeleton | Hs 99999903_m1 | 171 | 1 | 100 |
18 S | 18 S ribosomal | ribosomal subunit | Hs 03003631_g1 | 187 | 1 | 100 |
HPRT1 | Hypoxanthine- guanine phosphoribosyltransfe- rase | generation of purine nucleotides | F:ACAGGACTGAACGTCTTGCT R: GAGCACACAGAGGGCTACAA | 74 | 0.99 | 101.43 |
IFNG | Interferon gamma | immunoregulation | F: TCCAAGTGATGGCTGAACTGT R: TCGACCTCGAAACAGCATCT | 77 | 1 | 99.45 |
