Table 1 Molecular masses of recombinant TSPs and α2δ-1S NTST obtained by MALDI-TOF MS.

From: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4

Protein

m/zcalc [M + H]+

m/zexp [M + H]+

TSP-2 (monomer)

132,137

146,958

TSP-4 (monomer)

107,409

109,351

COMP (monomer)

84,714

88,463; 87,178

α2δ-1S NTST (−DTT)

123,000

152,236

α2δ-1S NTST (+DTT)

112,130*

152,258

  1. The m/zcalc [M + H]+ values for all proteins (in Da) were calculated based on their amino acid sequences using ExPASy Compute pI/Mw online tool, while the m/zexp [M + H]+ values (in Da) were obtained from the MALDI-TOF MS spectra of the respective proteins.
  2. * m/zcalc [M + H]+ of α2 NTST was calculated for the expected product of the DTT-mediated reduction of the disulphide bond between α2 and δ-1S in α2δ-1S NTST.
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