Table 2 Nucleotide sequences of the primers used.
From: The gene structure and hypervariability of the complete Penaeus monodon Dscam gene
Primer | Primer sequence (5′—3′) |
|---|---|
D-F16 | 5′ ATGGGCACTACCTATATG 3′ |
D-F24 | 5′ CTGATCTTCCCTCCCTTC 3′ |
D-R30 | 5′ CAAGATCGCGATAGTCAC 3′ |
Introns/Exons confirmation | |
D-Ig4-F | 5′ TCGAGACTGTGCTCACTGT 3′ |
D-Ig4-R | 5′ GTGTCAATGCGAAGAACAGC 3′ |
D-Ig6-F | 5′ TGCAGTTCACACAAATGATGGA 3′ |
D-Ig6-R | 5′ AACAATGAGGGTGCCATTG 3′ |
D-Ig8-F | 5′ CACGCTGGATTGTGGAAC 3′ |
D-Ig9-R | 5′ TTCCAAAAGCATTGGTAGCC 3′ |
D-gFNIII1-F | 5′ AGAACATGGCAGCTGTCTTG 3′ |
D-gFNIII3-R | 5′ TCACCACCTGATGTGAAAGC 3′ |
D-gFNIII6-F | 5′ GAAGCCTGAGATTCCTGAAG 3′ |
D-gE0-R | 5′ TCCAGTGAGAGTCAATGTAG 3′ |
D-EL1(A)-F | 5′ TCTCTGCTGTAGTCGTCATC 3′ |
D-EL1(B)-R | 5′ CTCAACATCGCAGTCTAAAG 3′ |
D-I-E1B-F | 5′ CCCAGTTATTGCCACTATCG 3′ |
D-I-E2-R | 5′ TAGCAAGTCTCCCTGGAATC 3′ |
D-I-E4-F | 5′ TCTGCCCTTATGCTACCTTC 3′ |
D-I-E5-R | 5′ GGGTATGGCCACAAGTATAG 3′ |
D-gE8-F | 5′ CCTCAACCCAGCAACCATAG 3′ |
D-gE9-R | 5′ CTCCATAATTACGCTGCACAAG 3′ |
D-gE9-F | 5′ AACCGTGGAAGCACCTCT 3′ |
D-gE10-R | 5′ TTGGTTTGTGCACAATGTCT 3′ |
D-gE11-F | 5′ CCAAGAGCACAGAGGAGATG 3′ |
D-gE12A-R | 5′ GGTCTTATTCAGTTTCCTCG 3′ |
