Table 1 Strains and plasmids used in this study.
Strain/cell line | Relevant genotype and phenotypea | Reference or source |
|---|---|---|
Cell lines | ||
EA.hy926 | Hybrid cell line derived by fusing human umbilical vein endothelial cells with the permanent human cell line A549 | |
HaCaT | ‘Human adult low calcium high temperature keratinocytes’, spontaneously immortalized human keratinocyte cell line | |
S. aureus | ||
DSM20231 | Serovar 3 type strain, ATCC12600 | |
DSM20231 mazEF | DSM20231 mazEF::lox72 | This study |
DSM20231 mazEF clpC | DSM20231 mazEF::lox72 clpC::erm(B); EmR | This study |
LS1 | Murine arthritis isolate | |
LS1 clpC | LS clpC::erm(B); EmR | This study |
Newman | Clinical isolate (ATCC 25904) | |
Newman clpC | Newman clpC::erm(B); EmR | This study |
PBM001 | DSM20231 clpC::erm(B); EmR | |
PBM001 clpC+ | PBM001 clpC::pBT_SACOL0570’, clpC+; TcR | This study |
RN4220 | Restriction negative, modification positive NCTC 8325-4 derivative | |
SH1000 | NCTC 8325-4 rsbU+ derivative | |
SH1000 clpC | SH1000 clpC::erm(B); EmR | This study |
Plasmids | ||
pBT | pBC-SK derivative harboring an 1.6-kb PCR fragment of tet(L) from pHY300PLK cloned into the Alw26I restriction site; TcR | |
pBT_clpC’ | pBT derivative harboring an 1-kb DNA fragment of the C-terminus of clpC; TcR | This study |
pBT2-mazEF-ko-ermB | pBT2 derivative harboring the PmazEF promoter and the transcriptional terminator downstream of mazEF and a lox flanked erythromycin resistance cassette replacing the mazE and mazF open reading frames. | |
