Figure 3

Knockdown of ALDH3A1 leads to a marked reduction in production of NADH and ATP by SNU-638 cells, along with a concomitant fall in acetyl-CoA levels. (a–c) Effect of ALDH3A1 siRNA treatment (40 nM for 48 h) on metabolites derived from various metabolic pathways in SNU-638 cells. (d–f) Effect of gossypol treatment (5 µM) on metabolites derived from various metabolic pathways in SNU-638 cells. Relative pool sizes of metabolites after ALDH3A1 siRNA treatment for 48 h or gossypol treatment for 24 h were assessed by targeted LC-MS/MS and GC-MS. Abbreviations: 3-phosphoglycerate (3PG); Glucose 6-phosphate (G6P); Fructose 6-phosphate (F6P); Ribose 5-phosphate (R5P); Fumarate (FUM); Malate (MAL). Data are expressed as the mean and standard deviation of three independent experiments. *p <0.05, **p <0.01, ***p <0.001.