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Figure 3

From: New insights on Pseudoalteromonas haloplanktis TAC125 genome organization and benchmarks of genome assembly applications using next and third generation sequencing technologies

Figure 3

ORFs analysis of pMtBL. (a) pMtBL map. The OriR is highlighted in black. Manually analysed putative ORFs are represented as thick arrows. (b) orf2 expression analysis using end-point RT-PCR. After total RNA extraction a cDNA was synthetized using the primer pMtBL_B7_rv specific for orf2. Then PCR reactions with primers pMtBL_A4_fw and pMtBL_B7_rv were performed on the cDNA obtained from total P. haloplanktis TAC125 RNA after growth in GG (lane 2) and TYP media (lane 3). The PCR reaction was also carried out directly on RNA extracted after growths in GG (lane 4) and TYP (lane 5) and on total P. haloplanktis TAC125 DNA (lane 6). The expected amplicon of <100 bp was obtained only in the reactions where either the cDNA (lanes 2 and 3) or the total bacterial DNA (lanes 6) were used as templates. Total RNA templates did not lead to any amplification demonstrating the absence of DNA cross-contamination (lanes 4 and 5). Lane 1, 1 kb NEB marker. Full-length gel is presented in Supplementary Fig. S7.

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