Figure 4

Schematic diagram of pMtBL derivative shuttle vectors and their segregational stability. (a) Overview of the extent of pMtBL regions included in each vector series. pGEM-T-MtBL encompasses the entire pMtBL plasmid; MAV was developed only introducing the psychrophilic OriR4. (b) Retention of plasmids representative of each family derived from pMtBL without antibiotic selection. Each experiment was carried out as biological duplicates.