Figure 3
Deletion of iPLA2γ enhances autophagy and polyubiquitination in adriamycin nephrosis. Glomeruli were isolated from control (Ctrl) and iPLA2γ KO mice 4 weeks after adriamycin administration. (a,c,e) Representative immunoblots. Uncropped immunoblots are presented in Supplementary Fig. 5. (b,d,f) Densitometric quantification. (a,b) Glomerular lysates were immunoblotted with antibodies to LC3 and p62. LC3-II/actin was increased and p62/actin was decreased in iPLA2γ KO mice. *P < 0.035 KO vs control (adriamycin); 5 mice per group. In panel b, glomerular LC3-II levels in 6 untreated (Untr) mice (2 control and 4 KO) and p62 levels in 3 untreated mice (1 control and 2 KO) are shown for comparison. (c,d) Glomerular lysates were immunoblotted with anti-ubiquitin antibody. *P = 0.005 KO vs control, 6 control mice and 5 KO mice. (e,f) Lysates were immunoblotted with antibodies to pAMPK and AMPK. Levels of AMPK were highly variable among mice, and while there was an upward trend, there was not a significant difference in pAMPK/AMPK between control and KO mice (6 mice per group).
