Figure 4
Role of iPLA2γ in basal and ER stress-induced changes in LC3 and pAMPK. WT and iPLA2γ KO GECs were untreated (Untr), or incubated with or without chloroquine (CQ, 25 µM) and tunicamycin (Tm, 5 µg/ml) for 18 h. Lysates were immunoblotted with antibodies to LC3 (a) or AMPK and pAMPK (b). (a,c) Representative immunoblots. Uncropped immunoblots are presented in Supplementary Fig. 5. (b,d) Densitometric quantification. (b) LC3-II/actin *P < 0.0001 KO vs WT (CQ + Tm), P = 0.08 KO vs WT (CQ). LC3-II/actin in KO/CQ was 146 ± 16% of WT/CQ (P < 0.01). 5 experiments performed in duplicate. (d) pAMPK/AMPK *P < 0.05 CQ + Tm vs Untr (WT), **P < 0.05 KO vs WT (Untr). 3 experiments performed in duplicate.
