Figure 5
Role of iPLA2γ in basal and ER stress-induced changes in LC3-II puncta. WT and iPLA2γ KO GECs were transfected with RFP-LC3, and were incubated with or without chloroquine (CQ, 25 µM) and tunicamycin (Tm, 5 µg/ml) for 18 h. Representative photomicrographs and quantification of LC3-II puncta are presented. Bar = 25 µm. Puncta count: *P < 0.001 CQ + Tm vs CQ (WT), **P < 0.05 KO vs WT (CQ). Puncta area: *P < 0.001 CQ + Tm vs CQ (WT). 18–28 cells per group in 2 experiments.
