Table 1 STR Markers included in the SureID 23comp Kit.

Chr.	SureID 23comp			Investigator HDplex STRs^(a,b)	PowerPlex CS7 STRs
Chr.	STRs^(a)	Location (GRCh38)	Repeat structure*	Investigator HDplex STRs^(a,b)	PowerPlex CS7 STRs
1	D1S1656^a	230769616–230769683	CCTA [TCTA]n		F13B
2	D2S441^a	68011947–68011994	[TCTA]n	D2S1360
3	D3S1744	147374752–147374828	[ATAG]n atg [ATAG]n at [ATAG]n	D3S1744
4	D4S2366	6483114–6483172	[GATA]n [GATT]n [GATA]n gac [GATA]n	D4S2366
5	D5S2800	59403132–59403199	[GGTA]n [GACA]n [GATA]n [GATT]n	D5S2500
6	D6S474	112557951–112558018	[AGAT]n [GATA]n	D6S474, SE33^b	F13A01
7	D7S3048	21227099–21227174	[TATC]n [TACC]n [CACC]n	D7S1517
8	D8S1132	106316692–106316774	[TCTA]n tca [TCTA]n	D8S1132	LPL
9	D9S1122	77073826–77073873	[TAGA]n		Penta C
10	D10S1248^a	129294244–129294295	[GGAA]n	D10S2325
11	D11S2368	19259601–19259684	[TATC]n [TGTC]n [TATC]n
12	D12S391^a	12297020–12297095	[AGAT]n [AGAC]n AGAT	D12S391^a
13	D13S325	42599304–42599382	[TCTA]n tca [TCTA]n
14	D14S1434	94842054–94842105	[CTGT]n [CTAT]n
15	D15S659	46081911–46081966	[TATC]n		FESFPS, Penta E
16	D16S539^a	86352702–86352745	[GATA]n
17	D17S1301	74684855–74684902	[AGAT]n
18	D18S1364	65732998–65733056	[TAGA]n TACA [TAGA]n	D18S51^a
19	D19S253	15617484–15617531	[ATCT]n
20	D20S482	4525692–4525747	[AGAT]n
21	D21S2055	39819508–39819649	[CTAT]n CTAA [CTAT]n (30n) [TATC]n	D21S2055	Penta D
22	D22GATA198B05	17169811–17169882	CTCT [ATCT]n [ACCT]n

This table shows the locations (GRCh38) and repeat structures of the 22 STRs included in the SureID 23comp Kit. Five loci are common with the CODIS and the ESS. Twelve loci are not included in other available supplementary kits (Investigator HDplex and PowerPlex CS7). All information was adopted from^17,18,39. As proposed by Phillips¹⁹, the D5S2500 locus name is corrected to D5S2800 (Please see Note S1).
^aCODIS and ESS locus.
^bGermany core locus.
*Lowercase and italic nucleotides are not counted in allele nomenclature system³⁹.

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