Figure 2

Application of DAMIAN to RNA-Seq libraries from diagnostic BAL samples from patients with viral respiratory infections. Donut shaped charts represent the distribution of host (grey) versus non-host (orange) reads. The pie chart illustrated the taxonomic classification of non-host reads; represented are the relative abundance of contigs assigned to these species. Reads not aligning to sequences in the NCBI database are indicated in black, bacterial sequences are represented in yellow, viral contaminants are shown in pink. The pathogen most likely contributing to the clinical symptoms is indicated in read. In each sample, the contigs of the putative pathogen identified in the sample are aligned to the closest relative: (A) Influenza A, H1N1 (full-length segments); (B) Influenza A, H3N2 (full-length segments); PIV3 and HSV-1.