Figure 5
TNF rapidly activates Cx43 hemichannel opening in EA.hy926 cells via a Ca2+-dependent mechanism. (a) Consecutive current traces obtained at −50 mV (60 s) with and without 100 ng/mL TNF in solitary EA.hy926 cells. TNF induced periodic burst opening of ~220 pS unitary current activity, which was blocked by 100 µM Gap19 or 10 mM BAPTA, and enhanced by 100 µM CT9 (applied via the pipette solution). (b) Inset with corresponding all-point histogram demonstrating unitary activity characterized by a ~220 pS conductance. (c) Qm summary data of a (ncells = 8–13 per condition; 6 independent experiments). Data are expressed as mean ± SEM. ‘#’ indicates significance as compared to baseline condition, two-tailed paired student t test was used. ‘*’ indicates significance versus control TNF recording (2nd bar), one-way ANOVA with Dunnett’s multiple comparison test was used.
