Figure 6

Ca²⁺ and CaMKII are implicated in TNF-induced renal vascular permeability. (a–c) Male C57BL/6J mice were pre-injected i.v. with vehicle (6% DMSO in DPBS) or BAPTA-AM (2 mM in DPBS) 30 min prior to i.v. injection of TNF (LD₈₀); control condition is BAPTA-AM injected (i.v., 2 mM) without TNF; n = total number of mice per group. (a,b) Cumulative survival rates and body temperatures presented as a function of time. Pool of 3 independent experiments; ^*p = 0.0324. (c) Vascular permeability shown as RLU of FITC-dextran in colon, ileum, lungs and kidneys 6 hr after injection of control (BAPTA-AM) or TNF. Pool of 2 independent experiments. Colon: ^**p = 0.0086, ^***p = 0.0003; Lungs: ^*p = 0.0469; Kidney: ^****p < 0.0001, ^*p = 0.0108; ^**p = 0.0017. (d) Male C57BL/6J mice were injected i.v. with vehicle (6% DMSO in DPBS) or KN-93 (10 mg/kg) 60 min prior to i.v. injection of TNF. Mice pre-treated with KN-93 are slightly (p = 0.069) protected against renal vascular leakage. The two-star difference equals a p-value of 0.0034. In (b–d), data are presented as mean ± SEM. The statistical tests used: (a) Mantel-Cox test; (b,c) 2-Way ANOVA with post-hoc Tukey’s test; (d) 1-Way ANOVA with post-hoc Tukey’s test.