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Figure 2

From: Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Figure 2

D. tsuruhatensis 11304 QSI activity was determined using colorimetric agar well diffusion assay with Chromobacterium violaceum CV026 as an indicator strain. (a) The activity of untreated control of D. tsuruhatensis 11304 overnight culture (1) and cell-free supernatant (2). The heat treatment at 100 °C for 30 min (3, 4) and 60 min (5, 6). D. tsuruhatensis 11304 overnight culture (3, 5) and cell-free supernatant (4, 6) retained QSI activity and shown to be thermostable. Proteinase K treatment did not affect QSI activity of D. tsuruhatensis 11304 overnight culture (7) and cell-free supernatant (8). Ethyl acetate extract of D. tsuruhatensis 11304 exhibits QSI activity (9). Proteinase K and LB growth medium with 0.5% DMSO (10, 11) are used as negative controls. (b) Quantitative data of these experiments obtained by measurement of inhibition zones of violacein production by Chromobacterium violaceum CV026.

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