Figure 3

The effect of the D. tsuruhatensis 11304 QSI extract on P. aeruginosa MMA83 biofilm formation ability measured by crystal violet staining. The D. tsuruhatensis 11304 QSI extract inhibits biofilm formation in a dose-dependent manner. P. aeruginosa MMA83 cell growth is represented by white (logCFU/ml), and biofilm formation by gray bars (OD₅₉₅). Numbers on x-axis represent two-fold serial dilutions of the D. tsuruhatensis 11304 QSI extract with the unit of measure being mg/ml. DMSO – bacterial culture with 0.5% v/v of DMSO that is the highest concentration of DMSO in experiment (used when 5 mg/ml of QSI extract was added). Data analysis by Student’s t- test demonstrates significant difference (*p < 0.05, **p < 0.01, ***p < 0.001) between the treated biofilms and the control (DMSO treated control). No significant difference in the cell growth between treated and untreated controls was observed.