Figure 2 | Scientific Reports

Figure 2

From: Development of a Staphylococcus aureus reporter strain with click beetle red luciferase for enhanced in vivo imaging of experimental bacteremia and mixed infections

Figure 2

In vitro bacterial growth curves and bioluminescence signals. AH4807 (lux), AH4775 (luc), AH4826 (lux + luc), USA300 LAC::lux, LAC4303 (lux) and Xen36 (lux) (all 1 × 104 CFU) were cultured in 96-well plates without (none) and with the addition of different concentrations (0.03–1.2 mg/240 µL TSB/well) of D-Luciferin for 0–14 hours and absorbance at 600 nm (A600) (A) and bioluminescent signals (photons/0.1 second acquisition time) (B) were measured every 5 minutes on a plate reader (BioTek H1 Synergy) (n = 4 replicates with 2 iterations).

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