Figure 4

In vivo BLI of lux versus luc in a S. aureus bacteremia mouse model. A high inoculum (1 × 10⁷ CFU, 20% lethal dose) of AH4826 (lux + luc) or LAC4303 (lux) was injected intravenously in mice (n = 3 mice/group). On day 3, in vivo BLI signals were acquired before (−5 minutes) and at the indicated time points (up to 65 minutes) after administration of D-Luciferin (150 mg/kg s.c.) (IVIS Lumina III, PerkinElmer). (A,D) Representative images of in vivo BLI of the ventral (A) and dorsal (D) sides of the mice. (B,E) In vivo BLI signals (photons/s) ± SEM of the ventral (B) and dorsal (E) sides of mice inoculated with AH4826 ± administration D-Luciferin (150 mg/kg s.c.). (C,F) In vivo BLI signals (photons/s) ± SEM of the ventral (C) and dorsal (F) sides of mice inoculated with AH4826 (lux + luc) or LAC4303 (lux) ± administration of D-Luciferin (150 mg/kg s.c.) at 15–25 minutes prior to imaging the mice. ^†P < 0.01 between indicated groups, as calculated by a 2-tailed Mann–Whitney U test (C,F).