Figure 2
From: PGC-1β Induces Susceptibility To Acetaminophen-Driven Acute Liver Failure
High fat diet fed mice treated with APAP display increase oxidative damage. Eight weeks old FVB/N mice fed with HFD or chow diet for 1 month were intraperitoneally injected with either APAP (300 mg/Kg body weight) or equal volume of saline as vehicle control. Liver tissues were collected 6 hours after injection. (A) Staining of the liver sections with 8-Oxo-DG, NITT, and HNE. (B) GSH determination on liver tissues. Relative PGC-1β expression (C), and of genes related to GSH metabolism, Gcl-c (D), G6pdh (E) and Nqo1 (F) were evaluated by Real Time qPCR. Relative Gene Expression was evaluated in liver specimens from different groups, using TBP as housekeeping gene. Comparison of different groups (n = 6 mice/group) was performed using Two-way ANOVA followed by Bonferroni post-test. Data from groups sharing the same lowercase letters were not significantly different, whereas data from groups with different case letters were significantly different (p < 0.001).
