Figure 5
From: ATP amplifies NADPH-dependent and -independent neutrophil extracellular trap formation
NADPH-dependent and independent NETosis in Panx1−/− BMDNs. (A) Representative kinetic of A23187 (1 μM) stimulation in WT and Panx1−/− neutrophils with SYTOX fluorescence over time. (B) Representative kinetic of PMA (50 nM) stimulation of WT and Panx1−/− neutrophils with SYTOX fluorescence over time. (C,D) Quantification of Maximal (Max) values in A23187 and PMA stimulations respectively (n = 13). (E,F) Quantification of Hill Slope indicating rate of reaction in A23187 and PMA stimulations respectively (n = 13). (G,H) Quantification of half-maximal time (ET50) in A23187 and PMA stimulations respectively.
