Table 1 Kinetic parameters obtained for M. tuberculosis CysA2 in comparison with TSTs and MSTs previously characterized in the literature.

From: Mycobacterium tuberculosis CysA2 is a dual sulfurtransferase with activity against thiosulfate and 3-mercaptopyruvate and interacts with mammalian cells

Enzymes

TST

Enzyme activity

Reference

Km

cyanide

(mM)

Km

thiosulfate

(mM)

Km

3-mp

(mM)

Vmax

(µmol.min−1.mg−1)

Mycobacterium tuberculosis CysA2

(Mtu0100)

1.66

2.97

—

0,008 × 10−3

This work

Arabdopsis thaliana

(ATIG16460)

—

7.2

5.4

—

58

Rattus norvergicus

(Rno25274)

—

4.4

2.6

1.1 × 104

59

Bos taurus Rhobov

(Bta280946)

0.063

7.0

—

600

12

Azotobacter vinelandii

RhdA

(AvCA607450)

8.7

1.0

—

1250

30

Pseudomonas aeruginosa

RhdA

(PaePA4956)

16

7.4

—

815

16

Fusarium solani

(Necha2_57025)

2

—

—

0.083

17

Trichoderma harzianum

(COG2897)

7

—

—

0.069

17

Mycobacterium tuberculosis CysA2

(Mtu01000)

9.11

—

7.02

0,0461 × 10−3

This work

Escherichia coli

SseA

(Ecob2521)

1.25

—

8.34

—

60

Homo sapiens

(Hsa4357)

130

—

350

417

37

Arabidopis thaliana

(ATIG79230)

—

—

11

2.4 × 103

58

Leishmania major

(LMJF050970)

—

10.9

345

0.2

32

Leishmania mexicana

(LMXM050970)

—

20.8

345

—

32

Rattus norvegicus

(Rno192172)

—

6.2 × 10

1.2

—

58

  1. MST1_Arabidopsis thaliana (AT1G79230); MST2_Arabidopsis thaliana (AT1G16460); TST_Rattus norvegicus Rno25274); MST Rattus norvegicus (Rno192172); Rhobov_Bos Taurus (Bta280946); RhdA_Azotobacter vinelandii (AvCA607450); RhdA_Pseudomonas aeruginosa RhdA (PaePA4956); MST_Leishmania major (LMJF050970); MST_Leishmania mexicana (LMXM050970); TST_Trichoderma harzianum (COG2897); TST_Fusarium solani (Necha2_57025). (—) not identified.
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