Figure 4 | Scientific Reports

Figure 4

From: Salt-free fractionation of complex isomeric mixtures of glycosaminoglycan oligosaccharides compatible with ESI-MS and microarray analysis

Figure 4

The extracted ion chromatograms of [M -4H]4− ion corresponding to AEAB-labeled [1, 2, 3, 8, 0, 1] from IPRP fractions. The EICs have shown that the derivatized hexasaccharide composition [1, 2, 3, 8, 0, 1] collected from IPRP in the 22 to 23 min fraction (top) eluted from the HILIC column around 27.1 min, while the same composition from the IPRP 25 to 26 min fraction (bottom) eluted around 28.0 min on HILIC. The IPRP 23 to 24 min fraction contains the shoulders of both isomer peaks in the IPRP chromatogram, and by HILIC (middle) both the 27.1 and the 28 min peak are partially resolved.

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