Figure 4

Validation of the pipetting-based immunoassay for enzymatic colour development. (a) Specificity test of the pipetting-based immunoassay for enzymatic colour development with other RNA viruses, BPV, PRRSV, hPIV1, DV 3, and 4. Standard deviation is indicated by error bar. (b) Detection of various subtype of influenza A viruses by pipetting-based immunoassay for enzymatic colour development: A/canine/Korea/01/2007(i), A/equine/Kyonggi/SA1/2011(ii), A/California/04/2009(iii), A/Puerto Rico/8/1934(iv), A/aquatic bird/Korea/CN2/2009(v), A/Chicken/Korea/MS96/96(vi), and A/swine/Korea/P17-4/2017(vii). The phylogenetic tree was generated by the maximum-likelihood method with 1,000 replicates of bootstrap sampling and the Jones-Taylor-Thornton (JTT) model using MEGA 6²¹. The various subtypes of influenza A viruses tested in this study are denoted by the black dots. (c) Application of the pipetting-based immunoassay for enzymatic colour development on the nasal swab samples of grow-finish pigs. (d) Application of the pipetting-based immunoassay for enzymatic colour development on the faecal samples of wild birds.