Figure 5 | Scientific Reports

Figure 5

From: Pipetting-based immunoassay for point-of-care testing: Application for detection of the influenza A virus

Figure 5The alternative text for this image may have been generated using AI.

Validation of the pipetting-based immunoassay for antigen capture. (a) Comparison of limits of detection among the pipetting-based immunoassay for enzymatic colour development, commercial lateral flow kit, and pipetting-based immunoassay for antigen capture plus commercial lateral flow kit. Ten-fold diluted influenza A virus, A/swine/Korea/P17-4/2017 isolate in PBS was tested. (b) Comparison of limits of detection between commercial lateral flow kit alone and pipetting-based immunoassay for antigen capture plus commercial lateral flow kit at the shortened incubation time for the sample reaction (10 min). (c) Comparison of detection capability between commercial lateral flow kit alone and pipetting-based immunoassay for antigen capture plus commercial lateral flow kit using faecal samples from wild birds.

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