Figure 4 | Scientific Reports

Figure 4

From: Correlative light and scanning electron microscopy (CLSEM) for analysis of bacterial infection of polarized epithelial cells

Figure 4

Correlation of Lifeact-eGFP Caco-2 BBe1 monolayer cells grown on mesh grid with carbon + formvar film facing upwards. (A) A mesh grid was inserted into the SEM carrousel holder with no identifiable areas in low magnification. Imaging was performed with low ETH 1.8 kV to avoid damage of cells. The monolayer grew homogeneously on C + F film without topographic differences. (B) Use of the STEM detector allowed to re-identify specific mesh holes. To visualize the metal mesh underlying the cellular monolayer, ETH was increased to 15 kV as short as possible to find landmarks in grid centre. The best recognizable mesh hole has a small notch in one corner (red square). (C) Area of interest was imaged by InLens SE detector, correctly identified due to landmarks by STEM detector in (D). Scale bars, (A,B) 1 mm, (C,D) 100 µm.

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