Figure 7
CLSEM of EPEC interaction with polarized epithelial cells. (A) Grid area with EPEC-infected (red) Lifeact-eGFP MDCK cells (green) imaged by SDCM, fixed 3 h p.i. with GA. Formation of EPEC clusters on the apical membrane with F-actin-enriched protrusions termed pedestals (light green spots), visible at sites of adhering bacteria. Processed samples were analysed by SEM using the InLens SE detector with EHT of 4 kV. Shape and distribution of EPEC clusters was used for defined correlation within relocated mesh holes. Selected ROI i-iv are indicated by yellow boxes. (B) SEM of clusters of EPEC at higher magnification of ROI i-iv. F-actin-enriched spots in SDCM modality correlate with pedestals visible in SEM modality (light green arrowheads). Scale bars, (A) 20 µm, (B) 2 µm.
