Figure 4

Growth, measured by the increase in protein concentration, and methane production by cells of M. mazei under conditions of either Pi-starvation or after overplus. (A) Cumulative methane production. (B) Protein concentration. Filled circles: Pi-starved/overplus; empty circles: Pi-replete control. Phosphate was added to the starved cultures on day 4. Error bars indicate ± s.d. of biological triplicates. Paired t-test was used to assess the difference between treatment and control in each sampling day. *p < 0.05; **p < 0.01.