Figure 6
From: DDR1 autophosphorylation is a result of aggregation into dense clusters
SIM images reveal DDR1-immunostain double-walled structures for cells stimulated with collagen for 60 minutes at 37 °C. COS-7 cells transiently expressing DDR1 were stimulated with collagen I for 10 or 60 minutes at 37 °C or left unstimulated, then incubated on ice with mAb 7A9, before fixation and secondary Ab staining. 3D-SIM images were acquired using a Zeiss ELRYA microscope; images are from one of 15 slices. Lower panels show magnified views of the boxed areas in the upper panels. Arrowheads in A indicate example structures wider than 200 nm (full width at half maximum). Scale bars, 30 μm (upper panels) or 5 μm (magnified images). At least 10 cells were imaged for each condition.
