Figure 5
hAEC secretion of inflammatory markers decreases over time, especially at higher seeding densities but is not significantly impacted by ECM niche. Levels of (A) VCAM-1 are significantly lower on day 4 than on day 2. Similarly, secretion of inflammatory markers (B) MCP-1 and (C) GM-CSF tended to be lower on day 4 of culture. On day 2 MCP-1 levels are significantly higher at 284 cells//mm2 seeding density but were similar to lower seeding densities on day 4, while the other biomarkers remain unaffected by seeding density. Data are presented as the mean values of biomarker concentration normalized to cellular proliferation, ± SD, n = 7 (****p < 0.0001, ***p < 0.001, **p < 0.01).
