Figure 6
From: Immunoproteomic analysis of Borrelia miyamotoi for the identification of serodiagnostic antigens
Evaluation of cross-reactive antibodies present in B. burgdorferi-infected CD1 mice against recombinant B. miyamotoi proteins (100 ng). Pooled sera collected at 14 dpi from CD1 mice exposed to B. burgdorferi (B31)-infected I. scapularis was assayed against B. miyamotoi recombinant proteins and an IgM (a) or IgG (b) antibody response observed. Serum was diluted 1:200 for immunoblotting. Molecular weight of proteins is expressed in kDa. P.l. = putative lipoprotein; U.p. = uncharacterized protein; Vlp = variable large protein; Vsp = variable small protein; GlpQ = glycerophosphodiester phosphodiesterase; B. m. lysate = B. miyamotoi whole cell lysate; B. b. lysate = B. burgdorferi whole cell lysate; Vector = empty pETite expression vector. Dashed lines demarcate where the gel has been cropped to exclude molecular weight markers and portions of blots. Uncropped blots can be viewed in Supplemental Fig. 2.
