Table 1 Protein identities corresponding to immunoreactive spots.

From: Immunoproteomic analysis of Borrelia miyamotoi for the identification of serodiagnostic antigens

GO terma

Spot no.

Protein ID

pIb

NCBI accession ID

Pred MW in kDac

No. of unique peptides

CCd: Membrane

 

1, 3, 10, 11, 22, 31, 32, 39, 40

VlpC2g

5.84

ALU64348.1

35

26, 5, 28, 24, 14, 5, 9, 4, 4

 

2

Vlp5g

5.73

AOW96300.1

35

2

 

2, 4, 5, 6, 7, 22, 33

VlpD2g

5.55

ALM31567.1

34*

3, 5, 9, 14, 5, 12, 4

 

4, 5, 6, 7, 9, 22, 33

VlpD8g

5.43

ALU64349.1

33*

29, 32, 22, 7, 5, 16, 7

 

4, 5, 6, 7, 9, 10, 22

VlpD9g

5.96

ALU64350.1

34*

2, 3, 5, 9, 18, 2, 2

 

4, 5, 6, 7, 22

Vlp15/16g

5.61

AOW96282.1

33*

5, 7, 7, 5, 3

 

4, 5, 6, 22

VlpD1g

5.62

ALM31565.1

34

4, 8, 11, 8

 

5, 6, 7, 9, 22

VlpD10g

5.77

ALU64352.1

33*

2, 3, 15, 4, 2

 

14, 16, 20, 33, 38

Vlpg

5.87

AOW96324.1

33*

10, 4, 7, 14, 2

 

22

Vlpg

5.89

AOW96302.1

34

5

 

6, 10, 11, 15, 16, 17, 20 25, 26, 27, 30, 31, 32, 34, 35, 36

Vsp1h

6.18

AJA67245.2

22

3, 3, 5, 8, 4, 4, 2, 11, 3, 4, 20, 5, 20, 6, 2, 11

 

11

HflK

5.79

AGT27198.1

35

8

 

11

HflC

9.52

AGT27199.1

37

8

 

23

DUF1640 domain-containing protein

5.42

AOW96294.1

18

3

 

18

Ribonuclease Y

7.65

AGT27464.1

58

3

CCd: flagellum associated

 

1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 21, 22, 30, 32, 33

Flagellin

5.36

AGT27144.1

35

11, 13, 9, 10, 12, 11, 12, 10, 11, 10, 10, 13, 11, 10, 12

 

6, 22, 33

Flagellar assembly protein-FliH

5.28

AGT27286.1

35

14, 3, 3

 

28

Flagellar protein

5.33

AGT27283.1

24

2

 

2, 11, 14

Flagellar protein

8.38

AGT27611.1

39

10, 2, 3

 

23

Flagellar hook assembly protein-FlgD

5.49

AGT27281.1

16

2

 

38

Flagellar basal-body rod protein-FlgC

8.92

AGT27290.1

17

2

 

40

Flagellar motor switch protein-FliM

5.09

AGT27275.1

39

2

MFe: phosphoric diester hydrolase activity

 

1, 2, 5, 6, 8, 11, 22

GlpQi

5.68

AGT27237.1

39

16, 10, 2, 13, 9, 11, 15

MFe: ATPase and ATP binding

 

1, 31

Phosphate ABC transporter substrate-binding protein

6.92

AGT27210.1

31

2, 13

 

2, 4, 5, 7, 8, 19, 22

60 kDa chaperonin

5.28

AGT27591.1

59

5, 10, 4, 2, 4, 3, 8

 

3, 18

ABC transporter substrate binding protein

6.11

AGT27324.1

61

32, 19

 

3, 18, 19

ABC transporter substrate binding protein

5.90

AGT27323.1

61

18, 12, 4

 

8, 21

Chaperone DnaK

5.00

AGT27477.1

69

7, 40

 

10, 11

ATP-dependent zinc metalloprotease-FtsH

8.61

AGT27731.1

71

2, 2

 

11

Holliday junction ATP-dependent DNA helicase-RuvB

7.04

AGT27027.1

38

2

 

11

ATPase

6.39

AGT27171.1

37

17

 

11, 20, 22

ABC-transporter binding protein

9.26

AGT27698.1

35

11, 2, 2

 

28, 29

V-type protein ATPase subunit E

5.49

AGT27099.1

23

10, 10

 

31

Phosphate import ATP-binding protein-PstB

6.22

AGT27213.1

29

9

 

39

ATP-dependent Clp protease ATP-binding subunit-ClpX

7.97

AGT27557.1

48

2

MFe: protein binding

 

8, 31

Uncharacterized protein

8.33

WP_082002168.1

30

2, 4

MFe: catalytic activity

      
 

31

Triosephosphate isomerase-TpiA

6.23

AGT27058.1

28

13

 

32

5’-methylthioadenosine/S-adenoslyhomocystein nucleosidase

5.97

WP_082583095.1

25

5

 

39

Pyruvate kinase

6.18

AGT27339.1

53

21

 

39

Inosine-5’-monophosphate dehydrogenase-GuaB

8.48

AJA67229.1

53

7

MFe: double-stranded RNA binding

 

11

Threonylcarbamoyl-AMP synthase

9.35

AGT27679.1

38

4

MFe: translation associated

 

15, 30, 32

50 S ribosomal protein-L25

6.77

AGT27728.1

21

3, 2, 6

 

18

Lysine-tRNA ligase

8.90

AGT27602.1

61

8

 

23, 25

30 S ribosomal protein-S10

10.05

AGT27452.1

12

2, 2

 

24

Elongation factor G

5.50

AGT27495.1

77

2

 

25

50 S ribosomal protein L21

9.70

AGT27721.1

12

4

 

26, 27

50 S ribosomal protein L5

9.60

AGT26990.1

20

2, 4

 

26, 27, 35

50 S ribosomal protein L9

9.62

AGT27115.1

19

4, 4, 2

 

32

50 S ribosomal protein L3

9.83

AGT26984.1

23

2

 

37

50 S ribosomal protein L19

10.23

AGT27642.1

17

2

 

38

30 S ribosomal protein S7

9.99

AGT26980.1

18

2

MFe: GTP-binding

 

7

Ribosome-binding ATPase YchF

5.40

AGT27227.1

41

3

BPf: metabolic processes

 

2

Ornithine carbamoyltransferase

5.74

WP_043867909.1

37

7

 

2

Phosphoglycerate kinase

5.84

AGT27059.1

43

4

 

4, 5, 22

Enolase

5.05

AGT27330.1

47

3, 2, 2

 

11, 14, 20, 33

GAPDHj

8.32

AGT27060.1

36

17, 3, 16, 2

BPf: cell morphogenesis

 

11

Rod shape-determining protein

6.86

AGT27659.1

38

17

BPf: proteolysis

      
 

10

Serine protease

8.99

WP_082002164.1

53

3

 

39

M18 family aminopeptidase

6.03

AGT27354.1

52

7

BPf: transcription associated

 

18

Transcription termination/antitermination protein-NusA

4.74

AGT27741.1

55

2

 

4, 5, 6, 22, 33

DNA-directed RNA polymerase subunit alpha-RpoA

5.11

AGT27462.1

38

17, 16, 15, 18, 3

 

28, 29

Transcription termination/antitermination protein-NusG

5.48

AGT27376.1

21

12, 6

BPf: polyamine binding

 

11

Spermidine/putrescine import ATP-binding protein

8.78

AGT27580.1

40

3

 

22

Sperimidine/putrescine ABC transporter substrate-binding protein

5.46

AGT27583.1

41

13

BPf: protein folding

 

32

Protein GrpE

5.38

AGT27478.1

21

4

BPf: chemotaxis

      
 

37, 38

Probable chemoreceptor glutamine deamidase-CheD

8.63

AGT27553.1

18

3, 4

Unassigned

 

4, 8, 12, 37

Uncharacterized protein

8.22

AGT27688.1

77

4, 2, 13, 2

 

6

Uncharacterized protein

5.63

AOW96351.1

35

6

 

8

Uncharacterized protein

5.18

AGT27657.1

30

5

 

11, 20

Uncharacterized protein

9.02

AGT27150.2

44

3, 4

 

11

Uncharacterized protein

6.15

AOW96199.1

37

2

 

11, 20

Uncharacterized protein

8.91

AGT27497.1

42

2, 2

 

13

Uncharacterized protein

8.80

AGT27550.1

67*

16

 

21

Uncharacterized protein

5.09

AOW96138.1

28

3

 

22

Uncharacterized protein

5.15

AOW96267.1

35

2

 

23

Uncharacterized protein

5.44

AGT27223.1

15

5

 

23

Uncharacterized protein

5.72

AOW96390.1

19

5

 

25

Uncharacterized protein

5.91

AOW96369.1

29

3

 

23, 24, 25

Uncharacterized protein

6.18

AOW96394.1

15*

8, 10, 6

 

29

Uncharacterized protein

8.37

AGT27607.1

25

5

 

30

Uncharacterized protein

6.86

AGT27219.1

28

4

 

32

Uncharacterized protein

7.66

AGT27434.1

27

2

 

35

Uncharacterized protein

6.21

AGT27719.1

18*

3

 

39

Uncharacterized protein

8.11

AGT27044.1

59

3

 

23

Heat-shock protein

5.72

WP_043867868.1

17

8

 

11

Carboxylesterase

9.53

AGT27588.1

38

2

 

16, 30, 36

Peptidoglycan-binding protein-LysM

8.52

AGT27318.1

44*

5, 2, 2,

 

4, 5, 6, 8, 11, 22

Putative lipoprotein

5.24

ALN43426.1

35

2, 3, 4, 3, 3, 13

  1. Proteins are categorized according to common gene ontology (GO) terms as listed in UniProtKB. Each spot number (no.) corresponds respectively to the no. of unique peptides detected during protein identification.
  2. aGO terms = gene ontology terms; bpI = isoelectric point; cPred MW = predicted molecular weight; dCC = cellular component; eMF = molecular function; fBP = biological process; gVlp = variable large protein; hVsp = Variable small protein; iGlpQ = glycerophosphodiester phosphodiesterase; jGAPDH = glyceraldehyde-3-phosphate dehydrogenase; * = predicted pI and MW excludes signal sequence if detected via ExPASy.
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