Figure 4 | Scientific Reports

Figure 4

From: Upregulation of caveolin-1 and its colocalization with cytokine receptors contributes to beta cell apoptosis

Figure 4The alternative text for this image may have been generated using AI.

Down-regulation of cav-1 expression in INS-1 and isolated islets reduced cytokine mixture-induced cell damage. (a) CON siRNA (10 μM) or Cav-1 siRNA (10 μM) were transfected in INS-1 cells for 24 h. Cell viability was measured via the MTT assay after 24 h treatment with the cytokine mixture (CM; IL-1β 20 ng/ml, TNFα 20 ng/ml). The islets were cultured for 24 h following isolation from male C57BL/6 J mice (b) and male SD rat (c). Isolated islets were treated as described in (a) and cell viability was measured via the MTT assay. Values are means ± SEM from triplicate experiments, *p < 0.05 vs. CON siRNA treated with CON, #p < 0.05 vs. CON siRNA treated with CM.

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