Figure 6
AQP4 distribution in the gastric pit (GP), fundic gland (FG), small intestinal gland (SIG), and colonic epithelium (CEP) in tissue sections from O. cuniculus (A–D) and L. yarkandensis (E–H). (A–H) Representative immunohistochemistry of the AQP4 distribution in the gastric pit, fundic gland, small intestinal gland, and colonic epithelium of O. cuniculus and L. yarkandensis. Paraffin sections (6 μm) of gastrointestinal tract tissue from O. cuniculus and L. yarkandensis. Sections were incubated with an anti-AQP4 antibody. Scale bar for A-H: 50 μm. (I–L) Densitometric analysis of all immunohistochemical results for the gastric pit, fundic gland, small intestinal gland, and colonic epithelium from O. cuniculus and L. yarkandensis (n = 6 animals per group). Ns: p > 0.05; *p < 0.05; **p < 0.01. In O. cuniculus, weak labelling was detected in the gastric pit (A), and strong labelling was detected in the parietal cells of the fundic gland (B) and small intestinal gland (C). In contrast, strong labelling was detected in surface mucous cells of the gastric pit (E) of L. yarkandensis, and weak labelling was detected in parietal cells of the fundic gland (F) and small intestinal gland (G). The densitometric values show higher expression levels of AQP4 in the gastric pit (I) of L. yarkandensis than in O. cuniculus and lower levels of AQP4 in parietal cells of the fundic gland (J) and small intestinal gland (K) of L. yarkandensis than in O. cuniculus.
