Figure 3 | Scientific Reports

Figure 3

From: CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)

Figure 3

Δ6abc/5Mt and Δ6bcMt accumulate 20:2n-6 and 20:3n-3 phospholipids in the liver when fed low LC-PUFA diet rich in 18:2n-6 and 18:3n-3. The wildtypes (WT), Δ6abc/5Mt and Δ6bcMt were fed low LC-PUFA and high LC-PUFA diets for 54 days. Phospholipids were separated on high performance thin layer chromatography silica gel 60 plates. Fatty acid methyl esters (FAMEs) were prepared by acid-catalyzed transesterification and quantified by gas chromatography coupled with mass spectroscopy. Results for tissue fatty acid composition are shown as mean ± standard deviation of liver samples from 3 fishes. Statistical differences between WT and CRISPR-mutated fish were determined using two-tailed t-test with unequal variance and are denoted as asterisks (*p ≤ 0.05 and **p < 0.01).

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