Figure 4 | Scientific Reports

Figure 4

From: CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)

Figure 4

The degree of 14C-18:3n-3 desaturation in hepatocytes shown as percentage of radioactivity recovered in 20:3n-3, a substrate for Δ8-desaturation, and the overall impact of CRISPR-induced mutations in Δ6abc/5Mt on n-3 PUFA synthesis shown as percentage radioactivity recovered in n-3 PUFAs** (18:4n-3, 20:4n-3, 20:5n-3 and 22:5n-3) determined individually but pooled together. Approximately 90% of radioactivity was recovered from 14C-18:3n-3 in all samples. Data represent mean ± standard deviation of 3 and 2 samples from WT and the Δ6abc/5Mt respectively.

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