Figure 5

Δ6abc/5^Mt showed low dietary LC-PUFA-induced expression of sterol regulatory element binding protein-1 (srebp-1) in the liver (A) and pyloric caeca (B). The Δ6abc/5^Mt showed no significant effect on the expression of srebp-2 (C and D), however, mRNA expression of srebp-2 was downregulated in the liver of Δ6bc^Mt when fed low LC-PUFA diet (C).The mRNA expression of srebp-1 and srebp-2 in CRISPR-mutated fish was determined relative to wildtypes (WT), with WT set to 1. The impact of the two diets on the expression of srebp-1 and srebp-2 was determined by measuring mRNA expression in WT fed low LC-PUFA diet relative to WT fed high dietary LC-PUFA, with WT fed high LC-PUFA diet set to 1. Differences in dietary LC-PUFA showed no significant effect on mRNA expression of srebp-1 (E). However, WT fed low LC-PUFA diet showed upregulated mRNA expression of srebp-2 in the liver (F). WT, Δ6abc/5^Mt and Δ6bc^Mt were fed low LC-PUFA and high LC-PUFA diets for 54 days. All qPCR data were analyzed using qBase^{+ 51} which determined statistical differences between WT and CRISPR-mutated fish and between WT under the two dietary regimens by unpaired Mann-Whitney test with two-sided significance. Data are presented as means ± confidence interval with N = 5 per dietary treatment. Normalization was performed using elongation factor 1α-b (ef1α-b). Statistical differences between WT and CRISPR-mutated fish are denoted as asterisks (*p < 0.05, **p < 0.01).