Table 1 Weights and lengths of experimental fish fed different dietary regimens.

From: CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)

 

Weight (g)

Length (cm)

Number of fish

Dietary treatment

Feeding duration (days)

WT**

≈85

72

Standard diet

227

Δ6abc/5Mt**/Δ6bcMt

≈49

72 each

Δ6abc/5Mt

85 ± 25a

19 ± 2a

36

DHA-rich diets

110

Δ6bcMt

104 ± 25b

20 ± 2b

36

WT

176 ± 34c

24 ± 2c

36

Δ6abc/5Mt

203 ± 51ac

27 ± 2

6

Low LC-PUFA diet

54

Δ6bcMt

281 ± 52b

30 ± 2

6

WT

250 ± 62ab

30 ± 5

6

Δ6abc/5Mt

171 ± 36c

26 ± 1

6

High LC-PUFA diet

54

Δ6bcMt

191 ± 69ac

27 ± 3

6

WT

241 ± 47ab

29 ± 1

6

  1. Shortly after hatching fish were fed a standard commercial diet followed by 22:6n-3 (DHA)-rich diets to enhance growth. Fish were subsequently fed two experimental diets, low LC-PUFA and high LC-PUFA diets. **Weights of WT and Δ6abc/5Mt were estimated, as experimental fish were weighed together in two groups or categories as Pit-tagged (Δ6bcMt) or untagged (WT + Δ6abc/5Mt). At the time of switching from standard diet to 22:6n-3 (DHA)-rich diets, we did not weigh Δ6abc/5Mt group separately but they were observed to have similar size as Δ6bcMt compared to WT. Weights and lengths of fish fed low and high LC-PUFA diets were analyzed by two-way ANOVA using dietary treatment and strain or genotype as experimental factors, followed by multiple comparisons of the means using Tukey HSD post-hoc test. Weights and lengths of fish fed DHA-rich diets were analyzed by one-way ANOVA using strain as experimental factor, followed by Tukey HSD post-hoc test. Different superscripts indicates statistical difference, p < 0.05.