Figure 2 | Scientific Reports

Figure 2

From: Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island

Figure 2The alternative text for this image may have been generated using AI.

Examples of FISH with both 18S rDNA (green) and telomeric DNA (red) loci (a–j) and C-banding heterochromatin (a′-j′) of bivalents in diakinesis for the following Podisma sapporensis: X0-1,-2 (a–c,a′–c′) and XY-2 (d,d′); X0-3 (e,e′) and XY-4 (f,f′); X0-4 (g,g′), Co-1 (h,h′,i,i′); X0-10 (j,j′). The marked bivalent/s (number) and sex chromosome/s indicate the presence of rDNA clusters compared with the C-banding pattern; hybridization signals and the position of C-bands vary in size/position between homologous chromosomes (compare with Table 1) which are marked with an asterisk (*); (a,c) the insert in the left corner shows heterozygous M4 chromosomes forming a loop, indicating a partial absence of homology. B - indicates a metacentric supernumerary chromosome without rDNA probes (b,c) and C-bands (b′,c′); the insert in the bottom left corner (c′) shows this B chromosome in spermatogonial metaphase. 3 h - indicate heterozygous bivalent M3 (a,a′,c,c′,d,d′,f,f′); t - translocation between some chromosomes (i,i′,j). White arrows showed the presence of interstitial hybridization signals (ITSs) in long/medium autosomes (b,c,g) or during translocation between some of the bivalents (j). X/XY, sex chromosome/s. Scale bar = 10 µm.

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