Figure 1
From: Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Structural characterization of peptide/siRNA complex. (a) Peptide sequences; “O” represents hydroxyproline, black is collagen domain, red is CPP domain, and green is FITC fluorescence tag. (b) ITC thermograms of V1/siRNA and V2/siRNA; calculated binding parameters were calculated assuming a single site independent model. (c) CD spectra of V1/siRNA complex at 25 °C and 37 °C; the same height of positive peak (λ = 220 nm) indicates folded (triple helical) conformation. (d) DLS size distribution by volume of V1N/siRNA complex; error bar represents standard deviation from six independent samples. (e) Serum stability assay: Both naked siRNA and V1N/siRNA complexes were incubated (variable times) with 10% human serum and separated in 20% native PAGE. The gel was stained with ethidium bromide. Lane 1, siRNA in MilliQ Water (red box); Lane 2, V1N/siRNA complex in MilliQ Water (red box); Lane 3, 5, 7, 9, 11 siRNA in serum; Lanes 4, 6, 8, 10, 12, V1N/siRNA complex in serum; Lane 13, serum; Lane 14, ladder. Image is a full-length gel.
