Table 1 Alleles of ALS-linked genes investigated in this study and their effect on viability when expressed either alone or in combination with Gem3^BART in muscle tissue.

From: SMN complex member Gemin3 self-interacts and has a functional relationship with ALS-linked proteins TDP-43, FUS and Sod1

ALS GENE	ALLELE	REF.	VIABILITY
ALS GENE	ALLELE	REF.	Mef2-GAL4>	Mef2-GAL4 > + Gem3^BART
Hs: *TDP-43; Dm:* *TBPH*	LOF: +/TBPH^Δ23	⁷³	Adult Viable	Adult Viable
	LOF¹: TBPH-RNAi [Trinity]	73	Adult Viable	Adult Viable
	LOF¹: TBPH-RNAi [Merton]	73	Adult Viable	Adult Viable
	LOF¹: TBPH-RNAi [Maudlin]	126	Death at P	Death at P
	OE²: hTDP-43^WT	72	Adult Viable	Death at P
	OE¹: hTDP-43^WT.GFP^#10	72	Death at P	Death at L3
	OE¹: hTDP-43^WT.GFP^#16	72	Death at P	Death at L3
	OE¹: hTDP-43^CTF.GFP^#14	72	Death prior to L3	N/A
	OE¹: Flag.hTDP-43^WT	73	Death at P	Death at L3
	OE¹: Flag.TBPH^WT	73	Death prior to L3	N/A
	OE²: TBPH^WT	75	Death prior to L3	N/A
	OE²: Venus-TBPH^WT	75	Death at P	Death prior to L3
Hs: **FUS; Dm: Caz**	LOF: caz¹/+*	75	Adult Viable	Adult Viable
	LOF²: caz-RNAi [Kellogg]	121,127	Adult Viable	Adult Viable
	LOF²: caz-RNAi [Oriel]	N/A	Adult Viable	Adult Viable
	OE²: Flag.caz^WT	75	Adult Viable	Death at P
	OE²: Flag.caz^P398L	75	Death at P	Death at P
	OE²: Flag.hFUS^WT	75	Adult Viable	Death prior to L3
	OE²: HA.hFUS^WT	128	Death at P	Death at P
	OE¹: hFUS^P525L-RFP.HA	129	Death prior to L3	N/A
	OE¹: hFUS^WT-RFP.HA	129	Death prior to L3	N/A
	OE¹: hFUS^R524S-RFP.HA	129	Death at P	Death at P
	OE²: Flag.hFUS^P525L	75	Death at P	Death at L3
Hs: *C9orf72*	OE²: G₄C₂-3	123	Adult Viable	Adult Viable
	OE²: G₄C₂-36	123	Adult Viable	Adult Viable
	OE²: GR-36	123	Adult Viable	Adult Viable
	OE²: GR-100	123	Death prior to L3	N/A
	OE²: PR-36	123	Adult Viable	Adult Viable
	OE²: PR-100	123	Adult Viable	Adult Viable
Hs: **SOD1; Dm: Sod1**	LOF: Sod1ⁿ¹/+*	80	Adult Viable	Adult Viable
	LOF²: Sod1-RNAi [Pembroke]	122	Adult Viable	Adult Viable
	LOF¹: Sod1-RNAi [Hertford]	122	Adult Viable	Adult Viable
	OE¹: hSOD1^WT	130	Adult Viable	Adult Viable
	OE²: hSOD1^WT. HA	131	Adult Viable	Adult Viable
	OE¹: hSOD1^G85R	130	Adult Viable	Adult Viable
	OE¹: hSOD1^A4V	130	Adult Viable	Adult Viable
	OE¹: Sod1	130	Adult Viable	Adult Viable

¹Transgenesis: random insertion; ²Transgenesis: Φ-C31 site-specific insertion; ^*Heterozygote; Hs, Homo sapiens (human); Dm, Drosophila melanogaster (fruit fly); N/A = Not Applicable; L3, third instar larval stage; P, pupal stage, LOF, loss of function; OE, overexpression.

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Table 1 Alleles of ALS-linked genes investigated in this study and their effect on viability when expressed either alone or in combination with Gem3^BART in muscle tissue.

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