Figure 3
From: Mucin CYS domain stiffens the mucus gel hindering bacteria and spermatozoa
Decreased cell motility in rCYSx12-enriched mucus scraped from mouse colons. (a) Study design for the analysis of motile cell displacements in mucus scraped from the colons of mice. Tracking and analysis were conducted with Fiji and Icy, respectively. (b) Representative trajectories of fluorescent S. enterica in the mucus from wild-type and transgenic mice. (c) Analysis of S. enterica linearity in mucus from wild-type and transgenic mice ex vivo using bean plots. (d) Analysis of S. enterica swimming speed in mucus from wild-type and transgenic mice ex vivo using bean plots. (e) Representative trajectories of spermatozoa in the mucus from wild-type and transgenic mice. (f) Analysis of spermatozoa linearity in mucus from wild-type and transgenic mice using bean plots. (g) Analysis of spermatozoa swimming speed in mucus from wild-type and transgenic mice using bean plots. For (c,d,f,g), and g, average and median are indicated by a horizontal bold line and a cross, respectively. The number of tracked cells (n) is indicated at the bottom of the figure and the statistics were conducted using a two-sided Wilcoxon-Mann-Whitney test. Results are representative of three independent experiments.
