Figure 7 | Scientific Reports

Figure 7

From: Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice

Figure 7

Distribution patterns of cortical granules in MII oocytes from wild type, heterozygous and mutant homozygous (hyh) mice. Confocal images of MII oocytes from different genotype after staining cortical granules (CG) with FITC-LCA and DNA with Hoescht 3342. Superficial plane: images were taken at the top surface to observe CG distribution (upper row). CG free domains can be clearly observed. Equatorial plane: images were acquired at the equatorial section in order to see chromatin and its position respect CG free domain of each oocyte (middle row). DIC. Differential interference contrast’s images show cell shape (purple dashed line; lower row). Scale bar: 20 μm.

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