Figure 3
From: Wedge prism approach for simultaneous multichannel microscopy
Wedge devices and testing. (a) Two-channel device installed in filter cube. (b) Two-channel false-color image of C. elegans neurons in vivo. (c) Four-channel device in emission filter housing. (d) Four-channel false-color image of red and blue-green beads. Red boxes outline channels. Lower left channel includes red, green, and blue light. (e) Diagram of green fluorescent bead PSF measurement without and with wedge device. (f–h) Resolution measurements under various objectives without (dashed) and with (solid) wedge device. FWHM values of PSF extent in x (yellow), y (blue), and z (green) are dependent on bead x position. Each point is the average of data from 40 beads. PSF FWHM from simulations indicated as points. Insets show simulated transverse PSF heatmaps at corresponding points.
