Figure 4
From: Wedge prism approach for simultaneous multichannel microscopy
Demonstration of approach in vivo and in fixed samples. (a) Single frame (0.2 s exposure) capture of RFP (red) and GCaMP (calcium-sensitive green) channel images of D-type motor axon in C. elegans in vivo prior to surgery at arrow (out of focus in frame). (b) Relative changes in green/red ratio reveal intracellular calcium dynamics at positions 1–3. Laser axotomy occurs at t = 2 s. Note delay and reduction in calcium transient with distance from surgery position. (c) Recombined image of electroporated chick embryo expressing Brainbow. Each neuron can be distinguished by a cell-specific ratio of fluorophores and intensity (normalized values).
