Figure 5

Index for PCR amplification efficiency of SSL-3. The index 2−Δ(ΔCt) was calculated based on the Ct values of SSL-3 and 18S ribosomal RNA genes, and indicates the relative amplification efficiency of the SSL-3 gene to that of standard strain Showa. (a) The 2−Δ(ΔCt) value was obtained for the 70 wild strains, and averaged by clades. Bars indicate standard errors. (b) The 2−Δ(ΔCt) values were averaged by climate regions where the wild strains were isolated. Filled circles indicate individual data of each strain, and rectangles are average values with standard error bars.