Table 1 Summary statistics for results of real-time PCR assay using SSL-3 and 18S ribosomal gene primers with DNA templates of Botryococcus braunii strains.

From: Detection of the oil-producing microalga Botryococcus braunii in natural freshwater environments by targeting the hydrocarbon biosynthesis gene SSL-3

Clade†

N

CtSSL3

Ct18S

ΔCt

2−Δ(ΔCt)

Average (SE)

Median

Max

Min

Average (SE)

Median

Max

Min

Average (SE)

Median

Max

Min

Average (SE)

Median

Max

Min

A

3

31.4 (0.85)

31.6

32.8

29.9

19.1 (1.3)

19.9

20.8

16.5

12.4 (1.8)

12.8

15.2

9.1

0.0075 (0.0065)

0.0015

0.021

0.00050

L

11

33.3 (0.42)

33.4

35.1

30.4

19.7 (0.12)

19.6

20.2

19.3

13.6 (0.40)

14.0

15.5

11.1

0.0020 (0.0005)

0.0011

0.0056

0.00040

S

7

33.3 (0.73)

33.9

35.7

30.0

19.5 (0.53)

19.5

22.4

18.2

13.8 (0.89)

13.6

17.6

10.6

0.0021 (0.0009)

0.0015

0.0073

0.000095

B1

12

25.0 (0.28)

25.0

26.5

23.4

20.1 (0.26)

20.2

21.6

18.9

4.9 (0.15)

5.0

5.4

3.6

0.60 (0.05)

0.57

0.92

0.36

B2

37

25.1 (0.24)

24.8

30.3

23.2

20.8 (0.25)

20.3

26.5

19.1

4.4 (0.06)

4.4

4.9

3.0

0.89 (0.03)

0.86

1.38

0.58

Showa§

 

23.5 (0.25)

 

19.3 (0.27)

 

4.2 (0.012)

 

1

 
  1. †Clade is classified based on the molecular phylogeny of 18S ribosomal sequence.
  2. §Average (SE) of Ct values with Showa DNA template (N = 3).
  3. ΔCt = CtSSL3 – Ct18S.
  4. Δ(ΔCt) = ΔCt –ΔCtShowa.
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