Table 2 List of primary and secondary antibodies used in this work.
Supplier, Cat # | Host | Dilution | Technique | |
|---|---|---|---|---|
Primary antibodies | ||||
anti-Iba-1 | Wako, 019–19741 | rabbit | (1:1000) | Microscopy |
anti-MHC-II | eBioscience, 14–5321–82 | rat | (1:500) | |
anti-Brn3a | Santa Cruz, sc-31984 | goat | (1:500) | |
anti-β-actin | Sicgen, AB0041-500 | goat | (1:1000) | Western blot |
anti-TNF | Abcam, ab66579 | rabbit | (1:1000) | |
anti-iNOS | Santa Cruz, sc-650 | rabbit | (1:500) | |
anti-IL-1β | R&D Systems, AF-401-NA | goat | (1:1000) | |
anti-PBR (TSPO) | Abcam, ab109497 | rabbit | (1:1000) | |
Secondary antibodies | ||||
Alexa Fluor® 488 Goat Anti-Rabbit IgG (H + L) | Invitrogen, A11008 | goat | (1:500) | Microscopy |
Alexa Fluor® 568 Goat Anti-Rat IgG (H + L) | Invitrogen, A11077 | goat | (1:500) | |
Alexa Flour® 568 Donkey Anti-Goat (H + L) | Invitrogen, A11057 | donkey | (1:500) | |
anti-rabbit IgG (H + L), HRP | BioRad,1706515 | goat | (1:10000) | Western blot |
HRP-coupled anti-goat IgG (H + L) | Invitrogen, LTI 611620 | rabbit | (1:10000) | |
AP-coupled anti-goat IgG (H + L) | Invitrogen, LTI 611622 | rabbit | (1:10000) | |
