Figure 2 | Scientific Reports

Figure 2

From: Induction of HLA-A2 restricted CD8 T cell responses against ApoB100 peptides does not affect atherosclerosis in a humanized mouse model

Figure 2

Peptide vaccination induces peptide responsive CD8 T cells. (A) Quantification of splenic CD8 T cell percentage. (B) Representative flow cytometry plots of splenic CD8 T cell activation by ApoB406–414 in the different treatment groups. (C) Quantification of IFN-γ and TNF-α double positive cells % from CD8 T cells. (D) Quantification of CD8 T cell percentage after ex vivo peptide stimulation mediastinal lymph nodes. (E) Representative flow cytometry plots of mediastinal lymph node CD8 T cell activation and (F) quantification of the IFN-γ and TNF-α double positive cell % from CD8 T cells after combined peptide stimulation. Statistical analysis was performed with 1-way ANOVA and Tukey’s multiple comparisons test. Depicted as mean with SEM, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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