Table 3 Composition of the three media used in this study.
Component | ASW | Medium 199 | M1 | |
|---|---|---|---|---|
g/L | ||||
Salts | NaCl | 23.300 | 6.800 | 15.420 |
Trizma HCL | 4.020 | — | 3.769 | |
Trizma Base | 2.970 | — | 2.784 | |
MgCl2 | 10.200 | — | 10.040 | |
Na2SO4 | 1.000 | — | 0.814 | |
CaCl2 | 1.000 | 0.200 | 0.400 | |
KCl | 1.000 | 0.400 | 0.302 | |
Amino Acids | L-Alanine | — | 0.025 | 0.053 |
L-Arginine · HCL | — | 0.070 | 0.095 | |
L-Aspartic Acid | — | 0.030 | 0.077 | |
L-Asparagine | — | — | 0.103 | |
L-Cystine · 2HCl | — | 0.000 | 0.000 | |
L-Cystine · HCl · H2O | — | 0.026 | 0.067 | |
L-Glutamic Acid | — | 0.067 | 0.114 | |
L-Glutamine | — | 0.100 | 0.163 | |
Glycine | — | 0.050 | 0.053 | |
Hydroxy-L-Proline | — | 0.010 | 0.010 | |
L-Histidine · HCl · H2O | — | 0.022 | 0.131 | |
L-Isoleucine | — | 0.020 | 0.070 | |
L-Leucine | — | 0.060 | 0.113 | |
L-Lysine · HCl | — | 0.070 | 0.089 | |
L-Methionine | — | 0.015 | 0.081 | |
L-Phenylalanine | — | 0.025 | 0.963 | |
L-Proline | — | 0.040 | 0.118 | |
L-Serine | — | 0.025 | 0.869 | |
L-Threonine | — | 0.030 | 0.055 | |
L-Tryptophan | — | 0.010 | 0.729 | |
L-Tryosine · 2Na · 2 H2O | — | 0.058 | 0.105 | |
L-Valine | — | 0.025 | 0.128 | |
* | Rifampicin | — | 0.030 | 0.030 |
Amphotericin B | — | 0.003 | 0.003 | |
