Figure 1
From: Quantification of the morphological characteristics of hESC colonies

(a) Phase-contrast image of a single isolated hESC at day 2 after plating, showing a well-defined nucleus, nucleoli (black dots) and spreading lamellipodia. Bar \(20\,\mu \)m. (b) Detail of the spatial arrangement of cells within a colony, with well-defined nuclei and prominent nucleoli (black dots). The very distinctive gaps between the cells occur in colonies with areas \(A < 0.6\,{\text{mm}}^{2}\). Bar \(50\ \mu \)m. (c) HESC colony in which the cells located at the top-left (outlined in blue) with larger nuclei. Bar \(100\ \mu \)m.